Single Cell Dna Amplification Kit
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Wga is achieved by pcr amplification of the library molecules using universal oligonucleotide primers.
Single cell dna amplification kit. Error bars indicate standard deviation for 8 21 replicates. Single cell genomiphi dna amplification kit has been optimized to wholly amplify genomic dna from as little as a single cell generating micrograms of high quality dna for use in downstream applications. The nebnext single cell low input cdna synthesis amplification module or the smart seq v4 ultra low input rna kit for sequencing clontech 634891 were used. At very low levels of input dna successful amplification relies on reagent quality.
The kit includes all reagents required for cell lysis whole genome amplification enzymatic dna fragmentation and pcr free ngs library preparation. Traditional single cell whole genome amplification methods yield insufficient quantities with significant biased representation. Cdna libraries were amplified using the number of pcr cycles shown. The genomeplex single cell wga kit wga4 containing the wga dna polymerase accurately amplifies plant dna as shown in the two model systems arabidopsis and tobacco.
The repli g advanced dna single cell kit is an improved version of the repli g single cell kit and includes an optimized reaction chemistry a new single cell storage buffer and an improved protocol to increase the uniformity of amplification and reduce potential amplification bias. Whole genome amplification from a single cell is now possible with our optimized genomeplex single cell whole genome amplification kit wga4. The genomeplex single cell whole genome amplification kit wga4 is designed to amplify the genome of a single cell resulting in a million fold amplification yielding microgram quantities of genomic dna. Genomeplex single cell whole genome amplification kit utilizes a proprietary technology based on random fragmentation of genomic dna and conversion of the resulting small fragments to pcr amplifiable library molecules flanked by universal priming sites.
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